Located near the Lycurgan cross-wall that separated the ancient sacred site from the main city of Eleusis, this small museum is the result of a large archaeological excavation.

Based on designs by architect Ioannis Moussisa, the original five-room facility was expanded to six in 1892 to make room for the abundant archaeological discoveries being made in the area.

The museum exhibits an array of artefacts outlining the history of Eleusis, from 1900 BC to the early Christian era.